antibodies against ki 67  (Servicebio Inc)

Journal: Non-coding RNA Research

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma

doi: 10.1016/j.ncrna.2026.03.003

Figure Lengend Snippet: circSMAD4 depletion in macrophages restrains LUAD growth and metastasis in vivo. (A) Schematic of orthotopic lung implantation and experimental metastasis models using LLC cells mixed with BMDMs expressing shNC or sh-circSMAD4. (B) Representative images of orthotopic lung tumors. (C) Tumor weight of orthotopic implants. (D) Overall survival of mice bearing orthotopic tumors. (E) Immunofluorescence showing F4/80 and circSMAD4 signals in tumor tissues. Scale bar, 50 μm. (F, G) Representative Ki-67 IHC staining and quantification in orthotopic tumors. Scale bar, 50 μm. (H) Representative bioluminescence images of lung tumor burden in the metastasis model. (I) Tumor weight in the metastasis model. (J) Overall survival of mice in the metastasis model. (K–M) Representative IHC staining and quantification of E-cadherin and vimentin in tumors. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499 ), E-cadherin (Proteintech, Cat# 20874-1-AP), and Vimentin (Proteintech, Cat# 10366-1-AP).

Techniques: In Vivo, Expressing, Immunofluorescence, Immunohistochemistry

Journal: iScience

Article Title: Optogenetic manipulation of estrogen receptor signaling to improve estrogen deficiency

doi: 10.1016/j.isci.2026.115105

Figure Lengend Snippet: OptoER relieved vaginal atrophy in ovariectomized (OVX) mice (A) Schematic timeline for the in vivo animal experiment. Adult female mice received a vaginal injection of AAV vectors (AAV-mCherry or AAV-OptoER). One week after viral delivery, mice underwent ovariectomy (OVX) to induce an estrogen-deprived state. Twenty days after OVX, light stimulation was applied, and tissue samples were collected for subsequent analysis. (B) Representative images of H&E-stained vaginal sections from different groups: OVX-Ctrl, OVX-OptoER, OVX-E2, and Sham mice. The results showed improved epithelial thickness and cell density in OVX-OptoER mice. (C) Representative images of PAS-stained sections from OVX-Ctrl, OVX-OptoER, OVX-E2, and Sham groups. Pink areas highlight glycogen-rich layers, indicating differences in the thickness of the cornified layer. (D) Representative images of Ki67-stained vaginal sections from OVX-Ctrl, OVX-OptoER, OVX-E2, and Sham mice. Brown staining indicates proliferating cells. Three mice were examined in each group. Scale bars, 50 μm.

Article Snippet: The sections were then incubated with a primary antibody against Ki67 (CST #12202), followed by a biotinylated secondary antibody and streptavidin-HRP complex.

Techniques: In Vivo, Injection, Staining